Preparation of genomic library (flowchart with description)
Solution:
DNA or genomic library is the total amount of DNA collected from a single organism.
Steps to prepare a genomic library are as follows:
DNA or genomic library is the total amount of DNA collected from a single organism.
Steps to prepare a genomic library are as follows:
- In order to prepare a genomic library, the organism's DNA needs to be extracted from the cell.
- Next, the DNA is digested with a restriction enzyme.
- This ensures that the DNA is cut into fragments of the specific size. The fragments are then inserted into the vector using DNA ligase.[1
- The fragments of DNA are collected and stored for their use in the molecular cloning.
- This DNA is stored in vectors containing various fragments of inserted DNA.
- These vectors are then maintained in the host cell mainly bacteria like E.coli.
- This allows easy amplification and extraction of the clones.