Is this statement correct - '' The enzyme alkaline phosphatase is used to remove the phosphate group from the 5' end of a DNA molecule, leaving a free 5' hydroxyl group.''
Shouldn't it be 3' hydroxyl group instead of 5' hydroxyl group since hydroxyl group is attached at 3' position of dna chain?
Hydroxyl group is present on both 3' and 5' end of the DNA molecule. Thus, the statement is true.